![]() 1988 Xing and Draper 1996 Holmberg and Noller 1999 Cameron et al. 1) must adopt an intricate tertiary structure to be functional ( Moazed et al. The 60 nucleotide (nt) GTPase center RNA (GAC) from 23S rRNA ( Fig. Previous Section Next Section INTRODUCTION The GTPase center RNA appears to have optimized its folding trajectory to specifically utilize this most abundant intracellular The sensitivity of RNA tertiary structure toĭivalent cation identity affects all but the fastest events in RNA folding, and allowed us to identify those states that prefer Rate constants for the fiveįolding transitions act on timescales from submillisecond to tens of seconds. In conformational ensembles along the folding pathway with transition times longer than 10 msec. Subsequently, specific divalent ions modulate populations of intermediates State with secondary structure to a more compact structure. Immediately upon addition of each divalent ion, the RNA undergoes a transition from an extended coli rRNA GTPase center, we combined stopped-flow fluorescence in the presence of Mg 2+, Ca 2+, or Sr 2+ together with time-resolved small angle X-ray scattering (SAXS) in the presence of Mg 2+ to observe the folding process. ![]() ![]() To measure how different divalent cations modify folding kinetics (nonspecific association) or binding (specific association). Folding of an RNA from secondary to tertiary structure often depends on divalent ions for efficient electrostatic charge screening ![]()
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